MagnetiQ Universal Pathogen DNA/RNA Extraction kit

The magnetiQ Pathogen Extraction Kit is designed to simplify the extraction of pathogenic DNA and RNA from a variety of sample types. Using buffers and magnetic beads that were optimized to capture DNA and RNA, users can obtain a purified product in 15 minutes.

A universal protocol for all pathogens from any matrix. The extraction of pathogenic DNA and RNA in only 4 steps, without the need for heating, centrifugation, vortexing or enzymatic/mechanical digestion. Using buffers and magnetic beads that were optimized to capture DNA and RNA, users can obtain a purified product in under 20 minutes.

The Universal Pathogen DNA/RNA Extraction Kit provides a safe, simple, and reproducible method for obtaining purified nucleic acid products. The kit can be used for downstream applications such as:

  • Detection of viral and bacterial infection

  • Pathogen population profiling

  • Quantification via PCR, RT-qPCR and digital PCR

  • Next Generation Sequencing (NGS)

  • Diagnosis, monitoring, and control of infectious diseases

  • Copy number variation (CNV)

Higher DNA/RNA yield from any pathogen/sample matrix

One-step magnetic bead-based extraction and purification of pathogenic DNA and RNA under 15 minutes from:

  • Swab solutions (VTM & ITM)

  • Serum/plasma

  • Blood and exudate

  • Saliva samples

  • Cell-free body fluids

  • Complex matrices such as sputum or stool

  • Cerebrospinal fluid (CSF)

FASTER TURNAROUND

More samples processed per day:

  • 20 minutes run time vs. 2+ hours manually

  • Prefilled plates – just add samples and go

  • Reduce backlog and accelerate reporting

    80% labour cost reduction

LOWER TOTAL COST OF OWNERSHIP

  • Ambient temperature shipping & storage = no spoilage losses.

  • Pre-loaded consumables = No additional plates or combs needed

  • Minimize manual error = Fewer failed runs and re-tests

Performance

MagnetiQ Universal Pathogen DNA/RNA Kit is benchmarked against industry leading kit:

• MagMAX™ Pathogen RNA/DNA Kit (ThermoFisher).

The comparison values between the ''Galenvs magnetiQ Universal Pathogen'' and "MagMAX™ Pathogen RNA/DNA Kit (ThermoFisher)'' kits were obtained following tests carried out by the Biovet Inc. laboratory.

Nasal samples – cows, pigs, pets - ΔCt values of quantitative PCR results comparing (i) Jupiter Universal Pathogen Kit and (ii) MagMAX™ Pathogen RNA/DNA Kit (ThermoFisher). Specimens include nasal samples containing bacterial and viral pathogens. ΔCt values above 0 represent a better DNA/RNA extraction efficiency of Jupiter Universal Pathogen Kit versus MagMAX™ Pathogen RNA/DNA Kit.

Oral samples – pigs, pets- ΔCt values of quantitative PCR results comparing (i) Jupiter Universal Pathogen Kit and (ii) MagMAX ™ Pathogen RNA/DNA Kit ThermoFisher). Specimens include oral samples containing bacterial and viral pathogens. ΔCt values above 0 represent a better DNA/RNA extraction efficiency of Jupiter Universal Pathogen Kit versus MagMAX™ Pathogen RNA/DNA Kit.

Fecal samples – cows, pigs, pets- ΔCt values of quantitative PCR results comparing (i) Jupiter Universal Pathogen Kit and (ii) MagMAX ™ Pathogen RNA/DNA Kit (ThermoFisher). Specimens include fecal samples containing parasites, bacterial and viral pathogens. ΔCt values above 0 represent a better DNA/RNA extraction efficiency of Jupiter Universal Pathogen Kit versus MagMAX™ Pathogen RNA/DNA Kit.

Gram-Positive Bacteria detection- Comparison of the performance of the Jupiter Universal Pathogen Kit versus MagMAX™ Pathogen RNA/DNA Kit (ThermoFisher) for the detection of the gram-positive bacteria Trueperella pyogenes. (A) Range of Ct values obtained for multiple samples using quantitative PCR for Trueperella pyogenes using Jupiter Universal Pathogen Kit and MagMAX™ Pathogen RNA/DNA Kit. (B) Percent of positively detected pathogen from the same collection of specimen samples (n=12) using Jupiter Universal Pathogen Kit and MagMAX™ Pathogen RNA/DNA Kit for T. pyogenes. A lower Ct value indicates a more sensitive and efficient purification of pathogenic nucleic acids.

Gram-Negative Bacteria detection- Comparison of the performance of the Jupiter Universal Pathogen Kit versus MagMAX™ Pathogen RNA/DNA Kit (ThermoFisher) for the detection of the gram-negative bacteria Pasteurella multocida. (A) Range of Ct values obtained for multiple samples using quantitative PCR for Pasteurella multocida using Jupiter Universal Pathogen Kit and MagMAX™ Pathogen RNA/DNA Kit. (B) Percent of positively detected pathogen from the same collection of specimen samples (n=17) using Jupiter Universal Pathogen Kit and MagMAX™ Pathogen RNA/DNA Kit for T. pyogenes. A lower Ct value indicates a more sensitive and efficient purification of pathogenic nucleic acids.

Parasite detection- Comparison of the performance of the Jupiter Universal Pathogen Kit versus MagMAX™ Pathogen RNA/DNA Kit (ThermoFisher) for the detection of the parasite M.Felis. (A) Range of Ct values obtained for multiple samples using quantitative PCR for M.Felis using Jupiter Universal Pathogen Kit and MagMAX™ Pathogen RNA/DNA Kit. (B) Percent of positively detected pathogen from the same collection of specimen samples (n=6) using Jupiter Universal Pathogen Kit and MagMAX™ Pathogen RNA/DNA Kit for M.Felis. A lower Ct value indicates a more sensitive and efficient purification of pathogenic nucleic acids.

Detection of PRRS (Porcine Reproductive and Respiratory Syndrome) from different matrices

Porcine Reproductive and Respiratory Syndrome (PRRS) is considered a major challenge in the veterinary industry due to its significant impact on the swine industry. The disease affects the reproductive and respiratory systems of pigs, leading to decreased productivity and increased mortality. This results in sgnificant economic losses for pig farmers and the industry. The management and control of PRRS is therefore of utmost importance to the veterinary industry.

Detection of PRRS- Comparison of the performance of the Jupiter Universal Pathogen Kit versus MagMAX™ Pathogen RNA/DNA Kit (ThermoFisher) for the detection of PRRS (Porcine Reproductive and Respiratory Syndrome) for multiple samples and from different matrices (oral fluid, tongue fluid, serum, and lungs), using quantitative PCR.

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Pathogen purification for high DNA & RNA recovery and quality of varying sizes

  • Short and scalable protocol

  • Rapid and cost-effective extractions

  • Non-toxic chemicals

  • Automatable on open liquid handling systems

Available in a range of formats designed to meet different sample size and throughput needs and designed for both manual and fully automated protocols.

Available in 50- and 200-prep manual extraction formats.

Also available in prefilled plate formats for automated extraction of pathogen DNA and RNA

  • Universal Pathogen DNA / RNA Extraction Kit

    The magnetiQ Universal Pathogen Extraction kit enables researchers to obtain a pure, contaminant free product. Its chemistry is optimized to lyse cells and wash away protein, lipid, salt and any other contaminants to yield a high purity sample. Our lysis buffer disrupts the components of the matrix enabling our magnetic beads to specifically target nucleic acids, which are subsequently purified from contaminants by the washing buffers and eluted in the final step of the process.  The Pathogen Extraction kit has been optimized for 200 µl of sample from which the DNA or RNA is eluted in 50-100 µl, however there is some flexibility in the protocol. Researchers have the capacity to use a range of sample from 100-400 µl and to increase the concentration of the product by eluting in volumes as low as 50 µl. DNA and RNA can be recovered from initial concentrations as low as 1 copy/µl.

    The magnetic beads used in the lysis buffer were developed to bind to DNA as well as RNA of different sizes with high specificity, without the use of Carrier RNA. This eliminates the need for any centrifugation step, filters, requires a minimal amount of collection tubes and can be easily applied to high throughput applications. 

    It does not require any additives such as Proteinase K or RNA carrier.

  • Universal Pathogen DNA / RNA Extraction Kit

    The magnetiQ Pathogen Extraction Kit has buffers optimized for pathogenic RNA and DNA capture. The functionalized magnetic beads in the lysis buffer were developed to target and bind nucleic acids specifically. All the components are stored at room temperature.

    Sample Type:

    Swab, Saliva, Serum / Plasma, Blood, Stool, Urine, and Various transport media such as Universal Viral Transport medium (VTM), Inactive Transport Medium (ITM), Exudate, CSF

    Formats Manual: 50 or 200 assays
    Automated: 16 assays

    Elution Volume: 50-100 µL

    DNA recovered>99%

    ProcessingMode: Automated; Manual. Compatible with 96-well plates.

    Throughput: 1-250 samples per run

    Binding Technology: Magnetic beads

    Binding Capacity: Scalable

    Components:

    (i) Pathogen Lysis/Binding Buffer
    (ii) Binding Magnetic Nanoparticles
    (iii) Wash Buffer #1
    (iv) Wash Buffer #2
    (v) Elution Buffer

    Storage: Room temperature

  • Guides and protocols